The following sections describe the procedure starting from electrotransfer of proteins to the membrane.įollow the links below for the methods used to generate antibodies, and the principle and method of SDS-PAGE. The bound enzyme activity is used to detect the target protein and visualized by a chemiluminescent or chromogenic method. The membrane with transferred proteins is then probed with a primary antibody (an antibody specific for the target protein), washed, and reacted with a secondary antibody labeled with an enzyme, such as horseradish peroxidase (HRP). In Western blotting (WB), target proteins are transferred to a hydrophobic membrane after SDS-PAGE and detected using specific antibodies.Īfter SDS-PAGE, a membrane is placed on the gel, to which the separated proteins in the gel are electrophoretically transferred. FAQs – In Vivo Isotype Control AntibodiesĪdvanced MHC Tetramers and Antibodies Searchįind products for Western blot The principle.Protein-Protein Interaction Detection Technology.FGF-Max (Recombinant human FGF-1/FGF-2 chimera).Afamin/Wnt3a CM and recombinant protein Show submenu.Non-Classical MHC Tetramers Show submenu.MHC Class I & Class II Tetramers Show submenu.SARS-CoV-2 MHC-Peptide Screening Kit Bundles. QuickSwitch™ Custom Tetramer Kits+ Show submenu.Custom MHC Tetramers and Monomers Order Form.You can rest easy knowing that Coker Tire Company is here to help you have the ultimate classic car experience. Coker Extended Tire Protection plan is a 5-year replacement program for tires that become unserviceable or damaged from a cut, snag, bruise, impact, sidewall damage, or puncture caused by a road hazard. Potholes, curbs, and road debris are everywhere and inevitably one of those unforeseen road hazards can leave you with a tire in need of repair or replacement. Dimensions for tires listed are not scientifically measured and may vary depending on rim widths and inflation. All tires and tubes should be installed by properly trained individuals. Coker Tire does not offer any guarantee of fitment for any particular purpose and limits its liability to the purchase price of the item. Coker Tire is not responsible for tire and tube failure due to improper mounting, mechanical failure or improper alignment of the vehicle. Adjustment is made on a prorated basis (you pay for tread wear used). If you want brand-name rubber that has been remade with modern technologies, make Coker your ultimate tire choice.Ĭoker Tire wants you to be pleased with your purchase! Coker tires are guaranteed to be free from defects in workmanship and materials for the life of the tread. Using antique designs and modern-day rubber, Coker Tire Company makes exceptional tires that look old and act like new. Today Coker is the leader in vintage tread representing Firestone, BG Goodrich, Michelin and other famous vintage tire styles. Coker Tire appeared on the automotive aftermarket scene in 1958 and has been building the vintage tire market since then. So keep your nostalgia racer, classic cruiser, or a street rod looking authentic all the way down with a set of reproduction tires from Coker Tire.Ĭoker Tire® are not the newcomers in the vintage cars' world. You need vintage-looking tires that perform like their modern counterparts. You need something great, something outstanding to complete the authentic look of your car. But you don’t need just a regular set of tires. And we're sure you’re not one of those collectible car owners who prefer to keep their classic cars in an enclosed garage 24/7 – we know that you want to show off your treasure to the world. Your car is your child, and you’ll do everything to keep it in tiptop shape. You make sure the paint is shiny, the engine runs perfectly, and the interior is spotless.
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